银杏
化学
色谱法
高效液相色谱法
银杏
检出限
葡萄糖苷
银杏
洗脱
梯度洗脱
校准曲线
乙腈
生药学
植物
生物化学
病理
生物活性
体外
替代医学
生物
医学
作者
Teruki Yoshimura,Nobuyoshi Udaka,Junsuke Morita,Jinyu Zhang,Keiko Sasaki,Daisuke Kobayashi,Keiji Wada,Yasushi Hori
标识
DOI:10.1080/10826070500531466
摘要
Abstract A method for simultaneous determination of ginkgotoxin (4′‐O‐methylpyridoxin), ginkgotoxin‐5′‐glucoside, and vitamin B6 compounds in Ginkgo biloba seeds, has been developed by using high performance liquid chromatography (HPLC) with fluorescent detection. Satisfactory separation was accomplished on a C18 reversed‐phase column by gradient elution with potassium phosphate containing sodium 1‐pentanesulfonate (pH 2.5)‐acetonitrile within 20 min. The linearity of the calibration curves for ginkgotoxin and ginkgotoxin‐5′‐glucoside ranged from 0.1 pmol to 10 pmol per injection, and the detection limit was 0.025 pmol (SN=5). Hot water extracts of raw, canned, and plastic packaged Ginkgo biloba seeds obtained in Japan were analyzed by using this method.
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