胆固醇
间充质干细胞
运行x2
甾醇O-酰基转移酶
碱性磷酸酶
化学
胆固醇酯
骨形态发生蛋白2
内分泌学
胆固醇逆向转运
内科学
细胞生物学
生物化学
生物
酶
体外
脂蛋白
医学
作者
Haifang Li,Hengjun Guo,Li Han
出处
期刊:Steroids
[Elsevier]
日期:2013-04-01
卷期号:78 (4): 426-433
被引量:24
标识
DOI:10.1016/j.steroids.2013.01.007
摘要
Effects of cholesterol on osteoblastic differentiation was evaluated in mouse bone marrow derived mesenchymal stem cells (MSCs). Cholesterol-treated MSCs showed a stimulated differentiation process with induced mRNA and protein levels of osteogenic lineage markers, increased alkaline phosphatase (AKP) activity and more mineralized nodules. However, the stimulation extent was reduced when incubating the cells with cholesterol plus the ACAT (acyl-CoA:cholesterol acyltransferase) inhibitor Sandoz58035 or SiRNA-ACAT1 [which blocks the esterification of free cholesterol (FC) to cholesteryl ester (CE)], indicating the osteogenic potency of cholesterol was mostly due to CE levels. The key role of BMP2 and Runx2 in the effects of cholesterol on MSC osteogenesis was elucidated. These results point to cholesterol as a modulator of osteoblastic differentiation, which separate cholesterol itself from other components of modified lipoproteins.
科研通智能强力驱动
Strongly Powered by AbleSci AI