Integrated cell metabolomics and serum metabolomics to reveal the mechanism of hypouricemic effect of Inonotus hispidus

As an easy recurrent metabolic disease, hyperuricemia affected the life quality of patients seriously. Inonotus hispidus is a kind of edible and medicinal fungi with hypouricemic effect. However, the mechanism remains unclear. In this study, cell metabolomics integrated with serum metabolomics were carried out to explore the potential mechanism of Inonotus hispidus in the treatment of hyperuricemia. The results indicate that the potential regulatory mechanism was mainly related to amino acid metabolism and glycerophospholipid metabolism, involving the regulation of 27 metabolites, including 12 in HHL-5 human primary hepatocytes and 15 in mouse plasma. Inonotus hispidus may show efficacy by regulating the synthesis of uric acid, and the fungi can also regulate the lipid disturbances caused by hyperuricemia. This work elucidated potential biomarkers and mechanisms of action in the hypouricemic effect of Inonotus hispidus from the perspective of endogenous metabolites, which provides a scientific basis for its further development and application.