Remote estimation of leaf nitrogen concentration in winter oilseed rape across growth stages and seasons by correcting for the canopy structural effect

Estimating crop leaf nitrogen concentration (LNC, %) with the canopy bidirectional reflectance factor (BRF) is an effective method for detecting the nitrogen (N) deficiency in crops. It is challenging to remotely estimate LNC across growth stages and seasons with a general empirical model, since the complex change in canopy structure under N deficiencies and across growth stages affects the accuracy of the estimations. The canopy scattering coefficient (CSC), the ratio of the BRF to the directional area scattering factor (DASF), has been suggested to reduce the canopy structural effect on BRF. However, the DASF can only be calculated for closed canopies and is not applicable to the early growth stages of crops when the fields are sparsely vegetated. This study proposed a new method for decoupling the canopy structural effect and canopy BRF using the near-infrared reflectance of vegetation (NIRV). NIRV is driven by the change in canopy structure while mitigates the soil contribution. The method was tested through six field experiments on ten farmers' fields of winter oilseed rape (Brassica napus L.) using both in-situ hyperspectral data and unmanned aerial vehicle (UAV) multispectral images. The results demonstrated that NIRV was closely related to the leaf area index (LAI) (R2 = 0.79) across growth stages and seasons. The CSC was derived with NIRV based on the linear relationship between NIRV and DASF for the closed canopies. The LNC predicted by the NIRV-derived CSC (R2 = 0.69, RMSE = 0.51 for in-situ hyperspectral data and R2 = 0.65, RMSE = 0.49 for UAV multispectral images) was more accurate than the results derived from the BRF (R2 = 0.55, RMSE = 0.62 for in-situ hyperspectral data and R2 = 0.59, RMSE = 0.60 for UAV multispectral images) with the independent dataset, suggesting that correcting for the canopy structural effect with NIRV provided a new alternative for suppressing the impact of canopy structure on the canopy BRF. As NIRV is easily calculated with diverse remote sensing data sources, this study proved the potential of applying NIRV to improving the accuracy and transferability of the LNC prediction model at different scales.