硫酸软骨蛋白多糖
神经周围网
细胞生物学
细胞外基质
再生(生物学)
轴突
硫酸软骨素
细胞内
化学
蛋白多糖
小胶质细胞
神经科学
脊髓
胶质瘢痕
脊髓损伤
炎症
生物
糖胺聚糖
生物化学
免疫学
作者
Xiumin Sun,Haiqian Liu,Zan Tan,Yuhui Hou,Mao Pang,Shengfeng Chen,Longyou Xiao,Qiuju Yuan,Limin Rong,Limin Rong,Liumin He
出处
期刊:Small
[Wiley]
日期:2022-11-18
卷期号:19 (6)
被引量:27
标识
DOI:10.1002/smll.202205012
摘要
The fluid-filled cystic cavity sealed by a dense scar developed following traumatic spinal cord injury (SCI) has been a major obstacle to neural regeneration and functional recovery. Here the transected lesion is bridged using a functional self-assembling peptide (F-SAP) hydrogel loaded with membrane-permeable intracellular sigma peptide (ISP) and intracellular LAR peptide (ILP), targeted at perturbing chondroitin sulfate proteoglycan (CSPG) inhibitory signaling. As compared to F-SAP hydrogel loaded with chondroitinase ABC, the F-SAP+ISP/ILP promotes a beneficial anti-inflammatory response via manipulation of microglia/macrophages infiltration and assembly of extracellular matrix (ECM) molecules into fibrotic matrix rather than scarring tissues. The remodeled ECM creates a permissive environment that supports axon regrowth and the formation of synaptic connections with neurons derived from endogenous neural stem cells. The remodeled networks contribute to functional recovery, as demonstrated by improved hind limb movements and electrophysiological properties. This work proposes a unique mechanism that ECM remodeling induced by CSPG-manipulation-based anti-inflammation can construct a permissive environment for neural regeneration, and shed light on the advancement of manipulation of cascading cellular and molecular events potential for endogenous repair of SCI.
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