Ultrasensitive Aptasensor for Microcystin-LR Detection in Food Samples Based on Target-Activated Assembly of Y-Shaped Hairpin Probes

As a kind of algal toxin, microcystin-LR (MC-LR) causes a tremendous treat to food safety and the detection of trace levels of MC-LR is highly desirable. Herein, we developed an ultrasensitive aptasensor for MC-LR detection based on target-activated assembly of Y-shaped hairpins. The aptamer-target recognition initiates the assembly step between two Y-shaped hairpin probes through toehold-mediated DNA replacement. One of the hairpins was modified with FAM and BHQ. Through cyclic assembly reactions, a high fluorescence signal can be observed in the product. The detection limit is 0.2 pM for MC-LR detection. In addition, the biosensor is robust and has been successfully explored to assess the MC-LR concentrations in real fish and water samples with satisfactory recovery rates and good accuracy. The signal amplification can be gained through the cyclic Y-shaped hairpin assembly, which offers a simple, ultrasensitive, and reliable method for MC-LR monitoring in food samples.