化学
DPPH
主成分分析
偏最小二乘回归
色谱法
化学计量学
抗氧化剂
分析化学(期刊)
生物化学
数学
统计
作者
Pei-Fei Gu,Ming Chen,Guoxiang Sun
标识
DOI:10.1016/j.foodchem.2024.139303
摘要
In this study, Black chokeberry (Aronia melanocarpa) was used as an example to provide reference for improving the safety, efficacy and quality consistency of homologous foods. In this study, two quality markers (Q-markers) of 27 batches of Black chokeberry were determined using high performance liquid chromatography (HPLC), and there were some differences among the 27 samples. Origin B samples had the highest levels of Q-markers for S15, and origin C had lower than average levels overall. Samples were analyzed qualitatively and quantitatively by Systematic Quantitative Fingerprinting (SQFM). Subsequently, a three-wavelength fusion analysis (TWFP) was established on the chromatographic data to compensate for the lack of a single wavelength. Fourteen batches of TWFP samples were rated at Level 5 or above in the SQFM assessment, indicating that there is some variation in the content of samples from different origins. Principal component analysis (PCA) was used to observe the differences in chemical composition and content of TWFP samples. Subsequently, electrochemical fingerprinting (ECFP) was established and nine characteristic parameters were recorded, showing that the samples were suppressed for all electrochemical Belousov-Zhabotinsky oscillation systems (B-Z oscillation systems). Finally, antioxidant tests were performed using DPPH. The antioxidant capacity was predicted using Partial Least Squares (PLS) analysis with R2Y = 0.84, Q2 = 0.77, a good model fit and accurate prediction. The fingerprint-potency relationship between IC50-peak area showed that 17 of the 19 shared peaks were negatively correlated, indicating that 17 peaks contributed significantly to the antioxidant. The methods established in this study for the determination of TWFP and ECFP, as well as the spectral relationships with peak area and IC50, can be used for the quality inspection and antioxidant capacity test of Black chokeberry, which provides a new research direction for improving the quality standard of medicinal and foodstuffs.
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