Characterization of thermostable recombinant laccase F from Trametes hirsuta and its application in delignification of rice straw

• Recombinant laccase F (rLacF) retained 93 % activity at 50 °C for 6 h. • Three-step hydrolysis process for sugar production from rice straw was established. • The rLacF pretreatment enhanced hydrolysis efficiency of hemicellulose . • The rLacF exhibited a good potential for further industrial application.