自愈水凝胶
透明质酸
明胶
细胞外基质
粘附
牛血清白蛋白
肿胀 的
机械转化
细胞粘附
材料科学
组织工程
生物物理学
生物医学工程
化学
细胞生物学
生物化学
高分子化学
解剖
复合材料
医学
生物
作者
Šimon Klimovič,Deborah Beckerová,Jakub Věžník,Daniil Kabanov,Karel Lacina,Šárka Jelínková,Jaromír Gumulec,Vladimír Rotrekl,Jan Přibyl
出处
期刊:Biomaterials advances
日期:2024-05-01
卷期号:159: 213819-213819
被引量:1
标识
DOI:10.1016/j.bioadv.2024.213819
摘要
Extracellular matrix (ECM) regulates cellular responses through mechanotransduction. The standard approach of in vitro culturing on plastic surfaces overlooks this phenomenon, so there is a need for biocompatible materials that exhibit adjustable mechanical and structural properties, promote cell adhesion and proliferation at low cost and for use in 2D or 3D cell cultures. This study presents a new tunable hydrogel system prepared from high-molecular hyaluronic acid (HA), Bovine serum albumin (BSA), and gelatin cross-linked using EDC/NHS. Hydrogels with Young's moduli (E) ranging from subunit to units of kilopascals were prepared by gradually increasing HA and BSA concentrations. Concentrated high-molecular HA network led to stiffer hydrogel with lower cluster size and swelling capacity. Medium and oxygen diffusion capability of all hydrogels showed they are suitable for 3D cell cultures. Mechanical and structural changes of mouse embryonic fibroblasts (MEFs) on hydrogels were compared with cells on standard cultivation surfaces. Experiments showed that hydrogels have suitable mechanical and cell adhesion capabilities, resulting in structural changes of actin filaments. Lastly, applying hydrogel for a more complex HL-1 cell line revealed improved mechanical and electrophysiological contractile properties.
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