丝素
丝绸
绿色荧光蛋白
控制释放
化学
生物物理学
药物输送
生物相容性材料
自愈水凝胶
化学工程
材料科学
生物医学工程
高分子化学
纳米技术
生物化学
有机化学
复合材料
生物
医学
工程类
基因
作者
Jaturong Promsuk,Juthatip Manissorn,Chavee Laomeephol,Jittima Amie Luckanagul,Apipon Methachittipan,Khaow Tonsomboon,Ratchapol Jenjob,Su‐Geun Yang,Peerapat Thongnuek,Kittikhun Wangkanont
标识
DOI:10.1038/s41598-024-53689-7
摘要
Abstract Controlled release of proteins, such as growth factors, from biocompatible silk fibroin (SF) hydrogel is valuable for its use in tissue engineering, drug delivery, and other biological systems. To achieve this, we introduced silk fibroin-mimetic peptides (SFMPs) with the repeating unit (GAGAGS) n . Using green fluorescent protein (GFP) as a model protein, our results showed that SFMPs did not affect the GFP function when conjugated to it. The SFMP-GFP conjugates incorporated into SF hydrogel did not change the gelation time and allowed for controlled release of the GFP. By varying the length of SFMPs, we were able to modulate the release rate, with longer SFMPs resulting in a slower release, both in water at room temperature and PBS at 37 °C. Furthermore, the SF hydrogel with the SFMPs showed greater strength and stiffness. The increased β-sheet fraction of the SF hydrogel, as revealed by FTIR analysis, explained the gel properties and protein release behavior. Our results suggest that the SFMPs effectively control protein release from SF hydrogel, with the potential to enhance its mechanical stability. The ability to modulate release rates by varying the SFMP length will benefit personalized and controlled protein delivery in various systems.
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