人巨细胞病毒
转录组
病毒学
巨细胞病毒
生物
细胞
计算生物学
基因
基因表达
遗传学
疱疹病毒科
病毒
病毒性疾病
作者
Michal Schwartz,Miri Shnayder,Aharon Nachshon,Tamar Arazi,Yaarit Kitsberg,Roi Levi Samia,Michael Lavi,Rottem Kuint,Reuven Tsabari,Noam Stern‐Ginossar
出处
期刊:Nature microbiology
日期:2023-02-02
卷期号:8 (3): 455-468
被引量:22
标识
DOI:10.1038/s41564-023-01325-x
摘要
Human cytomegalovirus (HCMV) can result in either productive or non-productive infection, with the latter potentially leading to viral latency. The molecular factors dictating these outcomes are poorly understood. Here we used single-cell transcriptomics to analyse HCMV infection progression in monocytes, which are latently infected, and macrophages, considered to be permissive for productive infection. We show that early viral gene expression levels, specifically of those encoding immediate early proteins IE1 and IE2, are a major factor dictating productive infection. We also revealed that intrinsic, not induced, host cell interferon-stimulated gene expression level is a main determinant of infection outcome. Intrinsic interferon-stimulated gene expression is downregulated with monocyte to macrophage differentiation, partially explaining increased macrophage susceptibility to productive HCMV infection. Furthermore, non-productive macrophages could reactivate, making them potential latent virus reservoirs. Overall, we decipher molecular features underlying HCMV infection outcomes and propose macrophages as a potential HCMV reservoir.
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