生物
基因
基因家族
遗传学
亚科
基因组
非生物胁迫
基因复制
拟南芥
AlkB
基因表达
蛋白质家族
DNA修复
突变体
作者
Jie Zhao,Tengfeng Yang,Lei Zhu,Huijie Liu,Hui Zhang,Sichao Guo,Xiaoye Liu,Xiaoguang Chen,Mingjia Chen
出处
期刊:Plants
[MDPI AG]
日期:2024-09-05
卷期号:13 (17): 2491-2491
标识
DOI:10.3390/plants13172491
摘要
Soybean (Glycine max (L.) Merr) is one of the most important crops worldwide, but its yield is vulnerable to abiotic stresses. In Arabidopsis, the AlkB homologue (ALKBH) family genes plays a crucial role in plant development and stress response. However, the identification and functions of its homologous genes in soybean remain obscured. Here, we identified a total of 22 ALKBH genes in soybean and classified them into seven subfamilies according to phylogenetic analysis. Gene duplication events among the family members and gene structure, conserved domains, and motifs of all candidate genes were analyzed. By comparing the changes in the m6A levels on mRNA from hair roots between soybean seedlings harboring the empty vector and those harboring the GmALKBH10B protein, we demonstrated that all four GmALKBH10B proteins are bona fide m6A RNA demethylases in vivo. Subcellular localization and expression patterns of the GmALKBH10B revealed that they might be functionally redundant. Furthermore, an analysis of cis-elements coupled with gene expression data demonstrated that GmALKBH10B subfamily genes, including GmALKBH10B1, GmALKBH10B2, GmALKBH10B3, and GmALKBH10B4, are likely involved in the cis-elements’ response to various environmental stimuli. In summary, our study is the first to report the genome-wide identification of GmALKBH family genes in soybean and to determine the function of GmALKBH10B proteins as m6A RNA demethylases, providing insights into GmALKBH10B genes in response to abiotic stresses.
科研通智能强力驱动
Strongly Powered by AbleSci AI