清脆的
核酸
生物传感器
核酸检测
计算生物学
生物技术
生化工程
纳米技术
生物
生物化学
工程类
材料科学
基因
作者
Yanan Sun,Tianjian Wen,Ping Zhang,Minglian Wang,Yuancong Xu
出处
期刊:Foods
[MDPI AG]
日期:2024-10-10
卷期号:13 (20): 3222-3222
标识
DOI:10.3390/foods13203222
摘要
Food safety is a major public health issue of global concern. In recent years, the CRISPR/Cas system has shown promise in the field of molecular detection. The system has been coupled with various nucleic acid amplification methods and combined with different signal output systems to develop a new generation of CRISPR/Cas-based nucleic acid biosensor technology. This review describes the design concept of the CRISPR/Cas-based nucleic acid biosensor and its application in food analysis. A detailed overview of different CRISPR/Cas systems, signal amplification methods, and signal output strategies is provided. CRISPR/Cas-based nucleic acid biosensors have the advantages of high sensitivity, strong specificity, and timeliness, achieving fast analysis of a variety of targets, including bacteria, toxins, metal ions, pesticides, veterinary drugs, and adulteration, promoting the development of rapid food safety detection technology. At the end, we also provide our outlook for the future development of CRISPR/Cas-based nucleic acid biosensors.
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