Wastewater treatment effectiveness is facilitated by crucial bacterial communities in the wetland ecosystem

Microorganisms play essential roles in nutrient removal and biogeochemical cycling during wastewater treatment. However, little is known about the main roles of key functional bacterial communities in wastewater treatment processes. We collected 18 water samples and 15 sediment samples from the six operational subsystems of the constructed wetland, among which the contact oxidation pond, enhanced hybrid biofilm reactor, and central stabilization pond are the main wastewater treatment units in the constructed wetland, and then investigated the bacterial communities using 16S rRNA gene targeting and sequencing to address this knowledge gap. The results indicated that the composition of the bacterial community is closely related to the efficiency of pollutant removal. The abundant carbon metabolism function increased the removal of nitrate‑nitrogen (NO 3 − -N) and total nitrogen (TN) by the contact oxidation pond by 89.84 % and 38.91 %, respectively. The overlap of ecological niches and the presence of pathogenic bacteria substantially affect effluent wastewater treatment. Second, NO 3 − -N ( p < 0.001) was the most important factor driving the bacterial community composition in water and sediments. Furthermore, the positive structure was prevalent in the cooccurrence network of water samples (87.24 %) and sediments (76.53 %) of the wetland, and this positive structure with keystone species was critical for the adaptation of the bacterial community to environmental filtration. In summary, this study reveals the distribution patterns of bacterial communities in different wastewater treatment processes and their driving factors and provides new perspectives on the link between the bacterial community composition and wastewater treatment. • NO 3 − -N drive the microbial composition of wetlands. • Carbon metabolism can improve wastewater treatment efficiency. • Pathogenic bacteria reduce bacterial community function. • Keystone species contribute to microbial community stability. • Positive structure improves community stability.