原肌球蛋白受体激酶B
细胞周期蛋白依赖激酶5
雪旺细胞
神经营养因子
细胞生物学
坐骨神经
神经营养素
周围神经损伤
脑源性神经营养因子
神经损伤
坐骨神经损伤
低亲和力神经生长因子受体
神经科学
神经生长因子
下调和上调
化学
生物
再生(生物学)
内科学
激酶
蛋白激酶A
解剖
医学
受体
细胞周期蛋白依赖激酶2
生物化学
基因
作者
Min Wen,Peng Li,Wenchao Bi,Ruize Yang,Y. Zhang
标识
DOI:10.1016/j.neulet.2023.137514
摘要
Cyclin-dependent kinase 5 regulatory subunit 1 (CDK5R1) is necessary for central nervous system development and neuronal migration. At present, there are few reports about the role of CDK5R1 in peripheral nerve injury, and these need to be further explored. The CCK-8 and EdU assay was performed to examine cell proliferation. The migration ability of Schwann cells was tested by the cell scratch test. The apoptosis of Schwann cells was detected by flow cytometry. Sciatic nerve injury model in rats was established by crush injury. The sciatic function index (SFI) and the paw withdrawal mechanical threshold (PWMT) were measured at different time points. The results revealed that overexpression of CDK5R1 promoted the proliferation and migration of Schwann cells, and inhibited the apoptosis. Further studies found that pcDNA3.1-CDK5R1 significantly upregulated the expression of CDK5, BDNF and TrkB. More importantly, CDK5R1 promoted the recovery of nerve injury in rats. In addition, the CDK5 mediated BDNF/TrkB pathway was involved in the molecular mechanism of CDK5R1 on Schwann cells. It is suggested that the mechanism by which CDK5R1 promotes functional recovery after sciatic nerve injury is by CDK5 mediated activation of BDNF/TrkB signaling pathways.
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