溶血磷脂酸
自交轴蛋白
滋养层
氧化应激
下调和上调
胎盘
信号转导
子痫前期
内分泌学
细胞生物学
内科学
化学
受体
生物
医学
生物化学
胎儿
怀孕
基因
遗传学
作者
Manami Yanagisawa,Takeshi Nagamatsu,Makoto Kurano,Fumi Misumi,Ayumi Taguchi,Naoya Akiba,Mari Ichinose,Keiichi Kumasawa,Takayuki Iriyama,Tomoyuki Fujii,Yutaka Yatomi,Yutaka Osuga
标识
DOI:10.1016/j.jri.2023.104153
摘要
The response of autotaxin (ATX)–lysophosphatidic acid (LPA) signaling system to placental oxidative stress (OS) and its significance to preeclampsia were investigated. For this purpose, oxidative stress index (OSI) and ATX levels were measured in the serum of pregnant women with preeclampsia. The expression levels of ATX and LPA receptors were assessed in trophoblast cells under high OS and glucose deprivation/re-oxygenation (OGD/R) conditions, with particular emphasis on the antioxidative nuclear factor erythroid 2-related factor 2 (NRF2) pathway. The influence of ATX–LPA signaling on cell migration was also evaluated using the wound healing assay. ATX concentrations and OSI in the serum were found to be elevated in preeclamptic women. The serum ATX levels were also positively correlated with OSI. Trophoblast cells responded to OS by increasing ATX mRNA expression concomitantly with intranuclear translocation of Nrf2, whereas inhibition of Nrf2 activation reverted this effect. The ATX–LPA signaling pathway facilitated trophoblast cell motility after Nrf2 activation. In conclusion, OS accumulation in preeclamptic placenta may activate the ATX–LPA system in trophoblasts via the Nrf2 pathway to sustain trophoblast functionality.
科研通智能强力驱动
Strongly Powered by AbleSci AI