Effect supplementation of black soldier fly larvae oil (Hermetia illucens L.) calcium salt on performance, blood biochemical profile, carcass characteristic, meat quality, and gene expression in fat metabolism broilers

This study evaluated the effect supplementation of black soldier fly larvae oil calcium salt (BSFLO-SCa) on performance, blood biochemical profile, carcass characteristic, meat quality, and gene expression in fat metabolism broiler chickens. A total 280 male New Lohmann strain MB 202 broiler chicks (one-day-old) were randomly placed into 4 treatments, including a control group (T0) were fed basal diet and a basal diet supplemented with 1% (T1), 2% (T2), and 3% (T3) BSFLO-SCa. Each treatment consisted of 7 pens with 10 chickens each. Results showed that 1% BSFLO-SCa supplementation significantly reduced (P<0.05) abdominal and meat fat, while gene expression on fat synthesis (FAS, ACC) were downregulated. Meat fatty acid profiles such as medium chain fatty acid being dominant in lauric and myristic and monosaturated fatty acid significantly increased (P<0.05). On the other hand, polyunsaturated fatty acid significantly decreased (P<0.05). In addition, the other parameters didn't affect by supplementation of 1% BSFLO-SCa. The addition starting from 2% significantly reduced (P<0.05) performance and carcass characteristics. Blood biochemical profiles (HDL, protein, albumin) and meat qualities (protein, cholesterol, water holding capacity, cooking losses, a*(redness) and b*(yellowness) values) were significantly increased (P<0.05), while gene expression on fat oxidation (CPT-1) was upregulated. In conclusion, broiler chicken that received of 1% BSFL-SCa does not negatively affect carcass performance and characteristics but reduced fattening in broiler meat.