血管平滑肌
表型
生物
流式细胞术
细胞
谱系标记
细胞生物学
谱系(遗传)
免疫组织化学
病理
平滑肌
分子生物学
免疫学
基因
医学
遗传学
内分泌学
作者
Alexander C. Bashore,Allen Chung,Chinyere Ibikunle,Hanying Yan,Chenyi Xue,Mingyao Li,Robert C. Bauer,Muredach P. Reilly
标识
DOI:10.1101/2023.09.29.557779
摘要
Abstract Vascular smooth muscle cells (VSMCs) play a central role in the development of atherosclerosis due in part to their capability to phenotypically transition into either a protective or harmful state. However, the ability to identify and trace VSMCs and their progeny in vivo is limited due to the lack of well-defined VSMC cell surface markers. Therefore, investigations into VSMC fate must utilize lineage-tracing mouse models, which are time-consuming and challenging to generate and not feasible in humans. Here, we employed CITE-seq to characterize the phenotypic expression of 119 cell surface proteins in mouse atherosclerosis. We found that CD200 is a highly expressed and specific marker of VSMCs, which persists even with phenotypic modulation. We validated our findings using a combination of flow cytometry, qPCR, and immunohistochemistry, all confirming that CD200 can identify and mark VSMCs and their derived cells in early to advanced mouse atherosclerotic lesions. Additionally, we describe a similar expression pattern of CD200 in human coronary and carotid atherosclerosis. Thus, our data support the use of CD200 as a lineage marker for VSMCs and VSMC-derived cells in mouse and human atherosclerosis.
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