强啡肽
偏头痛
微量注射
三叉神经脊核
疼痛
医学
咬肌
内分泌学
伤害
内科学
脑干
三叉神经节
神经病理性疼痛
麻醉
神经科学
阿片肽
类阿片
生物
解剖
受体
物理疗法
感觉系统
作者
Hui‐Kuo G. Shu,Sufang Liu,Joshua Crawford,Feng Tao
出处
期刊:Pain
[Ovid Technologies (Wolters Kluwer)]
日期:2023-07-18
卷期号:164 (12): 2801-2811
被引量:4
标识
DOI:10.1097/j.pain.0000000000002980
摘要
Abstract Migraine is commonly reported in patients with temporomandibular disorders (TMDs), but little is known about the mechanisms underlying the comorbid condition. Here, we prepared a mouse model to investigate this comorbidity, in which masseter muscle tendon ligation (MMTL) was performed to induce a myogenic TMD, and the pre-existing TMD enabled a subthreshold dose of nitroglycerin (NTG) to produce migraine-like pain in mice. RNA sequencing followed by real-time quantitative polymerase chain reaction confirmation showed that MMTL plus NTG treatment increased prodynorphin ( Pdyn ) mRNA expression in the spinal trigeminal nucleus caudalis (Sp5C) of female mice but not in male mice. Chemogenetic inhibition of Pdyn -expressing neurons or microinjection of antidynorphin antiserum in the Sp5C alleviated MMTL-induced masseter hypersensitivity and diminished the MMTL-enabled migraine-like pain in female mice but not in male mice. Moreover, chemogenetic activation of Pdyn -expressing neurons or microinjection of dynorphin A (1-17) peptide in the Sp5C enabled a subthreshold dose of NTG to induce migraine-like pain in female mice but not in male mice. Taken together, our results suggest that trigeminal dynorphin has a female-specific role in the modulation of comorbid TMDs and migraine.
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