An electrochemical aptasensor based on target triggered multiple-channel DNAzymes cycling amplification strategy with PtFe@Co-MOF as signal amplifier

脱氧核酶 适体 检出限 微分脉冲伏安法 材料科学 线性范围 纳米技术 电极 循环伏安法 电化学 化学 色谱法 遗传学 物理化学 生物
作者
Tong Zhu,Na Li,Jiangjian Huang,Xiaohansi Xu,Xin Su,Yi Ma,Renxiang Yang,Jia Ruan,Huilan Su
出处
期刊:Mikrochimica Acta [Springer Science+Business Media]
卷期号:189 (10) 被引量:16
标识
DOI:10.1007/s00604-022-05478-0
摘要

A novel electrochemical aptasensor for the detection of Aflatoxin B1 (AFB1) was developed for the first time by using the target-triggered multiple-channel deoxyribozymes (DNAzymes) cycling amplified assay with Pt Fe doped NH2-Co-MOF (PtFe@Co-MOF) as a signal amplifier. In the presence of AFB1, a self-assembling cross-over nucleic structure could be triggered by AFB1 via two aptamers’ structure switching for strand displacement, resulting in four channels of Mg2+-dependent DNAzyme recycling simultaneously to multiply the detection signals. These DNAzymes cyclically split the substrate sequence to release the PtFe@Co-MOF labeled detection probe (DP), which is subsequently hybridized with the capture probes on the Au-deposited glassy carbon electrode. The fabrication procedure was characterized by differential pulse voltammetry, and the results of the morphological and element composition characteristics methods were analyzed to determine the successful preparation of PtFe@Co-MOF. The limit of detection (LOD) for AFB1 detection was 2 pg mL−1 with a linear range from 5 pg mL−1 to 80 ng mL−1. By comparison, the enhanced detection sensitivity has been found to originate from the efficient shearing of DNAzymes, enhanced peroxidase-like capability, and multiple active sites of PtFe@Co-MOF. Besides, this aptasensor showed high specificity for AFB1 compared with similar mycotoxins and exhibited high accuracy with low experimental cost and easy operation. Furthermore, the unique design of electrochemical aptasensors could provide a promising platform for the onsite determination of AFB1, as well as other targets by replacing the aptamer and other core recognition sequences.Graphical abstract
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
李爱国应助甜甜发带采纳,获得10
刚刚
张宇姝完成签到,获得积分10
刚刚
Bran发布了新的文献求助10
刚刚
温热如河完成签到,获得积分20
刚刚
嘚嘚完成签到,获得积分10
1秒前
shilong.yang发布了新的文献求助10
1秒前
1秒前
斯文败类应助yyyyyge采纳,获得10
1秒前
qian完成签到,获得积分10
2秒前
2秒前
2秒前
2秒前
打打应助cslghe采纳,获得10
3秒前
hhuajw完成签到,获得积分10
3秒前
3秒前
3秒前
甜美板栗完成签到,获得积分10
3秒前
hmf1995完成签到 ,获得积分10
4秒前
失眠朋友发布了新的文献求助10
4秒前
ptiying发布了新的文献求助10
4秒前
轻松闭月完成签到 ,获得积分20
5秒前
Hathaway完成签到,获得积分10
5秒前
赘婿应助冷酷的珊珊采纳,获得10
5秒前
精明的沅应助美满花生采纳,获得10
5秒前
rrfhl发布了新的文献求助10
6秒前
丘比特应助文明8采纳,获得10
6秒前
所所应助叶95采纳,获得10
6秒前
6秒前
小二郎应助猪猪hero采纳,获得10
7秒前
深情安青应助小西贝采纳,获得10
7秒前
hdbys发布了新的文献求助10
7秒前
di123发布了新的文献求助10
7秒前
壮观的擎发布了新的文献求助10
7秒前
芮rich完成签到,获得积分10
7秒前
刺猬完成签到,获得积分10
8秒前
隐形曼青应助阮楷瑞采纳,获得10
8秒前
甜美板栗发布了新的文献求助10
8秒前
上官若男应助12w采纳,获得10
9秒前
9秒前
李健应助Minnie采纳,获得10
9秒前
高分求助中
Picture Books with Same-sex Parented Families: Unintentional Censorship 700
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
Effective Learning and Mental Wellbeing 300
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3974602
求助须知:如何正确求助?哪些是违规求助? 3519026
关于积分的说明 11196787
捐赠科研通 3255127
什么是DOI,文献DOI怎么找? 1797693
邀请新用户注册赠送积分活动 877100
科研通“疑难数据库(出版商)”最低求助积分说明 806130