仿形(计算机编程)
伤口愈合
组学
材料科学
医学
生物信息学
生物
免疫学
计算机科学
操作系统
作者
Shuaidong Chen,Chen-Yu Chu,Chenbing Wang,YANG YANG,Zhengyuan Xu,Yili Qu,Yi Man
出处
期刊:Biomaterials
[Elsevier]
日期:2024-12-01
卷期号:311: 122685-122685
标识
DOI:10.1016/j.biomaterials.2024.122685
摘要
Extracellular matrix (ECM) scaffold membranes have exhibited promising potential to better the outcomes of wound healing by creating a regenerative microenvironment around. However, when compared to the application in younger individuals, the performance of the same scaffold membrane in promoting re-epithelialization and collagen deposition was observed dissatisfying in aged mice. To comprehensively explore the mechanisms underlying this age-related disparity, we conducted the integrated analysis, combing single-cell RNA sequencing (scRNA-Seq) with spatial transcriptomics, and elucidated six functionally and spatially distinctive macrophage groups and lymphocytes surrounding the ECM scaffolds. Through intergroup comparative analysis and cell-cell communication, we characterized the dysfunction of Spp1+ macrophages in aged mice impeded the activation of the type Ⅱ immune response, thus inhibiting the repair ability of epidermal cells and fibroblasts around the ECM scaffolds. These findings contribute to a deeper understanding of biomaterial applications in varied physiological contexts, thereby paving the way for the development of precision-based biomaterials tailored specifically for aged individuals in future therapeutic strategies.
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