枯草芽孢杆菌
代谢组学
机制(生物学)
细胞生物学
发酵
计算生物学
化学
生物化学
生物
微生物学
细菌
生物信息学
遗传学
哲学
认识论
作者
Bo Zhang,Linlin Xu,Huiming Yang,Zhengjun Pang,Wenshuo Zhang,Fenghuan Wang,Huilin Liu,Junfeng Fan,Bolin Zhang
标识
DOI:10.1016/j.psep.2024.05.090
摘要
The cyclic lipopeptides (CPs) are synthesized by Bacillus spp. with excellent surface activities, but are limited in the application due to its low yield. More CPs secretion was previously determined in Bacillus subtilis culture under high cell density fermentation (HF). To explore the regulators and mechanism of sporulation and CPs, the proteomic and metabolomics profiles were firstly analyzed. As results, 95 different-regulated proteins were concentrated in TCA cycle, amino acid and fatty acid synthesis and metabolism pathways, promoting the synthesis of precursor peptide chains, inducing fatty acid transformation, and indirectly enhancing the formation of the CPs. Moreover, the correlation proteomic-metabolomics analysis showed that the surfactin and iturin yield were promoted by upregulation of NRPS synthetase (Sfp, Ste), while the downregulation of FenB (log2FC=-2.35) restricted the synthesis of fengycin. Besides, the addition of KH2PO4 increased the expression of two-component system (PhoR/PhoP), and promoted sporulation indirectly. Furthermore, sufficient substrate in HF system promoted energy metabolism pathway to produce more ATP and maintain cell vitality. Overall, our findings firstly provide a basis for further exploring potential regulation of CPs secretion induced by HF, and could be as a novel guild for look for the regulator of target substance in fermentation.
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