生物加工
球体
氧化铁纳米粒子
磁性纳米粒子
组织工程
纳米颗粒
材料科学
纳米技术
纳米材料
氧化铁
聚合物
脚手架
生物医学工程
化学
复合材料
生物化学
体外
冶金
医学
作者
Elizaveta V. Koudan,Mikhail N. Zharkov,М. В. Герасимов,Saida Sh. Karshieva,Aleksandra D. Shirshova,Vladimir V. Chrishtop,Vladimir Kasyanov,Aleksandr Levin,Vladimir Mironov,P. A. Karalkin,Frederico D. A. S. Pereira,Stanislav V. Petrov,N. A. Pyataev,Yusef D. Khesuani,Vladimir Mironov,Gleb B. Sukhorukov
出处
期刊:ACS Biomaterials Science & Engineering
[American Chemical Society]
日期:2021-10-05
卷期号:7 (11): 5206-5214
被引量:12
标识
DOI:10.1021/acsbiomaterials.1c00805
摘要
Magnetic tissue engineering is one of the rapidly emerging and promising directions of tissue engineering and biofabrication where the magnetic field is employed as temporal removal support or scaffold. Iron oxide nanoparticles are used to label living cells and provide the desired magnetic properties. Recently, polymer microcapsules loaded with iron oxide nanoparticles have been proposed as a novel approach to designing magnetic materials with high local concentrations. These microcapsules can be readily internalized and retained intracellularly for a long time in various types of cells. The low cytotoxicity of these microcapsules was previously shown in 2D cell culture. This paper has demonstrated that cells containing these nontoxic nanomaterials can form viable 3D tissue spheroids for the first time. The spheroids retained labeled fluorescent microcapsules with magnetic nanoparticles without a detectable cytotoxic effect. The high concentration of packed nanoparticles inside the microcapsules enables the evident magnetic properties of the labeled spheroids to be maintained. Finally, magnetic spheroids can be effectively used for magnetic patterning and biofabrication of tissue-engineering constructs.
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