Use of melatonin in sperm cryopreservation of farm animals: A brief review

Melatonin (MT) is a potent antioxidant with useful applications in several fields. Due to the capacity to scavenge free radicals and enhance cellular endogenous antioxidant defenses, MT is widely used in sperm cryopreservation to protect against oxidative stress-induced damage in frozen-thawed sperm. In this article, there is a review of positive effects of MT supplementation in cryopreservation of sperm from domestic ruminants and swine. There is direct or indirect scavenging of free radicals, preventing lipid peroxidation (LPO), and reducing oxidative stress, therefore, protecting membrane and DNA integrity, enhancing post-thaw antioxidant and enzymatic functions to maintain mitochondrial functions and activity, and regulating ATP production and utilization leading to maintenance of sperm quality, motility, and viability. In addition, MT reportedly inhibits sperm apoptosis, potentially by enhancing sperm viability and modulating abundances of mRNA transcripts.