各向异性
荧光各向异性
荧光团
粘度
荧光
激光诱导荧光
化学
分析化学(期刊)
材料科学
荧光素
微流控
温度测量
光学
热力学
纳米技术
色谱法
物理
复合材料
作者
Puneet Jain,Takuya Aida,Masahiro Motosuke
出处
期刊:Micromachines
[MDPI AG]
日期:2021-09-15
卷期号:12 (9): 1109-1109
被引量:6
摘要
Fluorescence anisotropy, a technique to study the folding state of proteins or affinity of ligands, is used in this present work as a temperature sensor, to measure the microfluidic temperature field, by adding fluorophore in the liquid. Fluorescein was used as a temperature-sensing probe, while glycerol–aq. ammonia solution was used as a working fluid. Fluorescence anisotropy of fluorescein was measured by varying various parameters. Apart from this, a comparison of fluorescence anisotropy and fluorescence intensity is also performed to demonstrate the validity of anisotropy to be applied in a microfluidic field with non-uniform liquid thickness. Viscosity dependence and temperature dependence on the anisotropy are also clarified; the results indicate an appropriate selection of relation between molecule size and viscosity is important to obtain a large temperature coefficient in anisotropy. Furthermore, a practical calibration procedure of the apparatus constant is proposed. In addition, the potential of temperature imaging is confirmed by the measurement of temperature distribution under focused laser heating.
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