Glucose activates the primordial follicle through the AMPK/mTOR signaling pathway

安普克 PI3K/AKT/mTOR通路 细胞生物学 卵泡发生 信号转导 内科学 内分泌学 卵泡 生物 葡萄糖摄取 河马信号通路 毛囊 化学 蛋白激酶A 磷酸化 卵巢 胰岛素 医学 胚胎 低温保存
作者
Shengyu Xu,Xiaoling Wu,Yanpeng Dong,Mengmeng Xu,Zimei Li,Sirun Chen,Yong Zhuo,Yan Lin,Lianqiang Che,Zhengfeng Fang,Bin Feng,Jian Li,Jianping Wang,De Wu,Zhihua Ren
出处
期刊:Clinical and translational medicine [Wiley]
卷期号:10 (3) 被引量:14
标识
DOI:10.1002/ctm2.122
摘要

Abstract Background We have previously found that the energy level in sows affects the activation of primordial follicles. Glucose is the primary metabolic substrate of dietary energy and its effect and mechanism of action with regards to the activation and development of primordial follicle remain unclear. Studies utilizing several different animal cells have shown that energy stress, induced by glucose starvation, activates AMPK and participates in a variety of cellular processes by regulating the Hippo and mTOR signaling pathways. However, whether glucose can affect primordial follicle activation through the above pathways has not been reported. Methods We developed an in vitro culture system for mouse ovaries to investigate the effects of glucose on the primordial follicle activation. Protein expression of AMPK‐Hippo‐YAP and AMPK‐mTOR pathway was investigated under glucose starvation and optimal glucose level treatment. Then, ovaries were treated with AICAR or Compound C in vitro to explore the effect of AMPK activation or inhibition on primordial follicle activation, and the changes of AMPK‐Hippo‐YAP and AMPK‐mTOR signaling pathways. Finally, investigated the signaling pathways affected by glucose potentially affecting the primordial follicle activation in vivo. Results The glucose was an essential nutrient for primordial follicle activation and we identified 25 mM glucose as the optimal level ( P < .05) for the primordial follicle activation in vitro. The glycolysis pathway was involved in primordial follicle activation ( P < .05) of ovaries cultured in vitro. The glucose affected the activation of primordial follicles in vitro through AMPK/mTOR signaling pathway by AMPK activation or inhibition treatment and follicle ratio count ( P < .05). Moreover, glucose affected the primordial follicle activation of ovary in vivo via mTOR signaling pathway. Conclusions This study demonstrates that glucose affects the primordial follicle activation through the AMPK/mTOR rather than the AMPK/Hippo signaling pathway.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
失眠夏之发布了新的文献求助10
刚刚
刚刚
刚刚
栗悟饭完成签到,获得积分10
1秒前
张开心完成签到 ,获得积分10
1秒前
欣慰的疾发布了新的文献求助10
2秒前
壮观千筹完成签到,获得积分20
3秒前
3秒前
小榕完成签到,获得积分10
3秒前
柒木发布了新的文献求助10
5秒前
香蕉觅云应助DY采纳,获得10
5秒前
光电发布了新的文献求助10
6秒前
6秒前
hoho完成签到,获得积分10
7秒前
xiaoliu发布了新的文献求助80
7秒前
7秒前
香蕉觅云应助欣慰的疾采纳,获得10
8秒前
毛毛毛发布了新的文献求助10
9秒前
机会完成签到,获得积分10
9秒前
10秒前
赘婿应助失眠夏之采纳,获得10
10秒前
z23150007发布了新的文献求助10
11秒前
唐宁发布了新的文献求助10
11秒前
现在就去看文献完成签到,获得积分10
11秒前
11秒前
dxx完成签到 ,获得积分10
13秒前
tong发布了新的文献求助10
14秒前
14秒前
麦子完成签到,获得积分20
14秒前
bela发布了新的文献求助10
15秒前
15秒前
隐形曼青应助暴躁的豆芽采纳,获得10
15秒前
16秒前
17秒前
wwwstt完成签到,获得积分10
17秒前
HYZ完成签到,获得积分10
18秒前
19秒前
19秒前
wwwstt发布了新的文献求助10
20秒前
相识发布了新的文献求助10
20秒前
高分求助中
좌파는 어떻게 좌파가 됐나:한국 급진노동운동의 형성과 궤적 2500
Sustainability in Tides Chemistry 1500
TM 5-855-1(Fundamentals of protective design for conventional weapons) 1000
CLSI EP47 Evaluation of Reagent Carryover Effects on Test Results, 1st Edition 800
Threaded Harmony: A Sustainable Approach to Fashion 799
Livre et militantisme : La Cité éditeur 1958-1967 500
Retention of title in secured transactions law from a creditor's perspective: A comparative analysis of selected (non-)functional approaches 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3054259
求助须知:如何正确求助?哪些是违规求助? 2711253
关于积分的说明 7425350
捐赠科研通 2355845
什么是DOI,文献DOI怎么找? 1247387
科研通“疑难数据库(出版商)”最低求助积分说明 606388
版权声明 596048