Glucose activates the primordial follicle through the AMPK/mTOR signaling pathway

安普克 PI3K/AKT/mTOR通路 细胞生物学 卵泡发生 信号转导 内科学 内分泌学 卵泡 生物 葡萄糖摄取 河马信号通路 毛囊 化学 蛋白激酶A 磷酸化 卵巢 胰岛素 医学 低温保存 胚胎
作者
Shengyu Xu,Xiaoling Wu,Yanpeng Dong,Mengmeng Xu,Zimei Li,Sirun Chen,Yong Zhuo,Yan Lin,Lianqiang Che,Zhengfeng Fang,Bin Feng,Jian Li,Jianping Wang,De Wu,Zhihua Ren
出处
期刊:Clinical and translational medicine [Wiley]
卷期号:10 (3) 被引量:14
标识
DOI:10.1002/ctm2.122
摘要

Abstract Background We have previously found that the energy level in sows affects the activation of primordial follicles. Glucose is the primary metabolic substrate of dietary energy and its effect and mechanism of action with regards to the activation and development of primordial follicle remain unclear. Studies utilizing several different animal cells have shown that energy stress, induced by glucose starvation, activates AMPK and participates in a variety of cellular processes by regulating the Hippo and mTOR signaling pathways. However, whether glucose can affect primordial follicle activation through the above pathways has not been reported. Methods We developed an in vitro culture system for mouse ovaries to investigate the effects of glucose on the primordial follicle activation. Protein expression of AMPK‐Hippo‐YAP and AMPK‐mTOR pathway was investigated under glucose starvation and optimal glucose level treatment. Then, ovaries were treated with AICAR or Compound C in vitro to explore the effect of AMPK activation or inhibition on primordial follicle activation, and the changes of AMPK‐Hippo‐YAP and AMPK‐mTOR signaling pathways. Finally, investigated the signaling pathways affected by glucose potentially affecting the primordial follicle activation in vivo. Results The glucose was an essential nutrient for primordial follicle activation and we identified 25 mM glucose as the optimal level ( P < .05) for the primordial follicle activation in vitro. The glycolysis pathway was involved in primordial follicle activation ( P < .05) of ovaries cultured in vitro. The glucose affected the activation of primordial follicles in vitro through AMPK/mTOR signaling pathway by AMPK activation or inhibition treatment and follicle ratio count ( P < .05). Moreover, glucose affected the primordial follicle activation of ovary in vivo via mTOR signaling pathway. Conclusions This study demonstrates that glucose affects the primordial follicle activation through the AMPK/mTOR rather than the AMPK/Hippo signaling pathway.
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