安普克
PI3K/AKT/mTOR通路
细胞生物学
卵泡发生
信号转导
内科学
内分泌学
卵泡
生物
葡萄糖摄取
河马信号通路
毛囊
化学
蛋白激酶A
磷酸化
卵巢
胰岛素
医学
胚胎
低温保存
作者
Shengyu Xu,Xiaoling Wu,Yanpeng Dong,Mengmeng Xu,Zimei Li,Sirun Chen,Yong Zhuo,Yan Lin,Lianqiang Che,Zhengfeng Fang,Bin Feng,Jian Li,Jianping Wang,De Wu,Zhihua Ren
摘要
Abstract Background We have previously found that the energy level in sows affects the activation of primordial follicles. Glucose is the primary metabolic substrate of dietary energy and its effect and mechanism of action with regards to the activation and development of primordial follicle remain unclear. Studies utilizing several different animal cells have shown that energy stress, induced by glucose starvation, activates AMPK and participates in a variety of cellular processes by regulating the Hippo and mTOR signaling pathways. However, whether glucose can affect primordial follicle activation through the above pathways has not been reported. Methods We developed an in vitro culture system for mouse ovaries to investigate the effects of glucose on the primordial follicle activation. Protein expression of AMPK‐Hippo‐YAP and AMPK‐mTOR pathway was investigated under glucose starvation and optimal glucose level treatment. Then, ovaries were treated with AICAR or Compound C in vitro to explore the effect of AMPK activation or inhibition on primordial follicle activation, and the changes of AMPK‐Hippo‐YAP and AMPK‐mTOR signaling pathways. Finally, investigated the signaling pathways affected by glucose potentially affecting the primordial follicle activation in vivo. Results The glucose was an essential nutrient for primordial follicle activation and we identified 25 mM glucose as the optimal level ( P < .05) for the primordial follicle activation in vitro. The glycolysis pathway was involved in primordial follicle activation ( P < .05) of ovaries cultured in vitro. The glucose affected the activation of primordial follicles in vitro through AMPK/mTOR signaling pathway by AMPK activation or inhibition treatment and follicle ratio count ( P < .05). Moreover, glucose affected the primordial follicle activation of ovary in vivo via mTOR signaling pathway. Conclusions This study demonstrates that glucose affects the primordial follicle activation through the AMPK/mTOR rather than the AMPK/Hippo signaling pathway.
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