色谱法
化学
组氨酸
分析物
毛细管电泳
检出限
组氨酸脱羧酶
作者
Akimitsu Kugimiya,Akane Fujikawa,Xiao Jiang,Z. Hugh Fan,Toshikazu Nishida,Jiro Kohda,Yasuhisa Nakano,Yu Takano
标识
DOI:10.1007/s12010-020-03365-z
摘要
A laminated paper-based analytical device (LPAD) for histidine detection was fabricated from a chromatography filtration paper and laminate films. Histidine recognition was effected by histidyl-tRNA synthetase (HisRS), and its detection was signaled colorimetrically based on the molybdenum blue reaction. The analytical conditions and detectable concentration range of histidine were examined. The method provided selective quantification from 1 to 100 μM histidine. LPAD fabrication is considerably simple, involving only the craft-cutting of the chromatography filtration paper and laminate film, and is cost-effective.
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