脑出血
p38丝裂原活化蛋白激酶
神经保护
MAPK/ERK通路
医学
药理学
四氯化碳
趋化因子
血脑屏障
CCR2型
炎症
信号转导
趋化因子受体
癌症研究
神经科学
细胞生物学
麻醉
内科学
中枢神经系统
生物
蛛网膜下腔出血
作者
Fuyou Guo,Dingkang Xu,Yu Cheng Lin,Guoqing Wang,Fang Wang,Qiang Gao,Qi Wei,Shixiong Lei
标识
DOI:10.1096/fj.201902203rr
摘要
Intracerebral hemorrhage (ICH) remains a devastating type of stroke that lacks an effective treatment. Recent evidence has demonstrated that CCL2 is involved in the blood-brain barrier (BBB) disruption and propagermanium (PG) as a CCL2 receptor inhibitor is neuroprotective in ischemic stroke. However, whether PG therapy exert effective role in acute ICH still unclear. In this study, our goal was to investigate the potential role of CCL2 and the effects of PG in ICH. Differentially expressed RNAs including CCL2 were detected in human ICH. CCL2 and the activation of p-p38 MAPK and AQP4 expression were analyzed in rats after ICH. Brain water content and BBB integrity as well as neurological function were also examined after PG administration. In addition, the mechanism by which CCL2-mediated BBB injury was further investigated by cell coculture. Our findings showed that PG could effectively reduce brain edema and improve neurobehavioral functions. p-p38 MAPK and AQP4 expression were significantly inhibited by PG in vivo and in vitro. To the best of our knowledge, this is the first demonstration of PG in neuroprotecting the BBB integrity by inhibition of CCL2-CCR2-p38 MAPK pathway following ICH, targeting CCL2 could be developed as a novel treatment for hemorrhagic stroke.
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