Application of phage therapy: Synergistic effect of phage EcSw (ΦEcSw) and antibiotic combination towards antibiotic‐resistant Escherichia coli

Transboundary and Emerging DiseasesVolume 67, Issue 6 p. 2809-2817 ORIGINAL ARTICLE Application of phage therapy: Synergistic effect of phage EcSw (ΦEcSw) and antibiotic combination towards antibiotic-resistant Escherichia coli Maheswaran Easwaran, Maheswaran Easwaran College of Veterinary Medicine, Chungnam National University, Daejeon, KoreaSearch for more papers by this authorMahanama De Zoysa, Mahanama De Zoysa College of Veterinary Medicine, Chungnam National University, Daejeon, Korea Research Institute of Veterinary Medicine, Chungnam National University, Daejeon, KoreaSearch for more papers by this authorHyun-Jin Shin, Corresponding Author Hyun-Jin Shin shin0089@cnu.ac.kr orcid.org/0000-0002-6805-4924 College of Veterinary Medicine, Chungnam National University, Daejeon, Korea Research Institute of Veterinary Medicine, Chungnam National University, Daejeon, Korea Correspondence Hyun-Jin Shin, College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungnam National University, Yuseong-gu, Daejeon, 34134, Korea. Email: shin0089@cnu.ac.krSearch for more papers by this author Maheswaran Easwaran, Maheswaran Easwaran College of Veterinary Medicine, Chungnam National University, Daejeon, KoreaSearch for more papers by this authorMahanama De Zoysa, Mahanama De Zoysa College of Veterinary Medicine, Chungnam National University, Daejeon, Korea Research Institute of Veterinary Medicine, Chungnam National University, Daejeon, KoreaSearch for more papers by this authorHyun-Jin Shin, Corresponding Author Hyun-Jin Shin shin0089@cnu.ac.kr orcid.org/0000-0002-6805-4924 College of Veterinary Medicine, Chungnam National University, Daejeon, Korea Research Institute of Veterinary Medicine, Chungnam National University, Daejeon, Korea Correspondence Hyun-Jin Shin, College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungnam National University, Yuseong-gu, Daejeon, 34134, Korea. Email: shin0089@cnu.ac.krSearch for more papers by this author First published: 26 May 2020 https://doi.org/10.1111/tbed.13646Citations: 2Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Bacteriophage therapy is acknowledged as a potential tool to prevent or treat multidrug-resistant bacterial infections. In this study, our major focus was on the bacteriolytic activity of phage EcSw (ΦEcSw) against the emergence of the clinically important Escherichia coli Sw1 and E. coli O157:H7. The amount of the antibiotics was changed in a concentration-dependent manner, and the ΦEcSw susceptibility to antibiotics was determined. The kanamycin and chloramphenicol inhibited the titre of phage, but ampicillin did not show phage inhibition. Though the kanamycin and chloramphenicol controlled the growth of Sw1 in a concentration-dependent manner, the ampicillin did not due to the resistance. The combined activity of the ΦEcSw with antibiotics (kanamycin and chloramphenicol) compared with the antibiotics alone showed significant lytic activity p < .001). In addition, phage-based therapy was evaluated for controlling the multidrug-resistant E. coli Sw1 and E. coli O157:H7 in zebrafish and BALB/c mice, respectively. Our results provide novel advantages of phage therapy and phage–antibiotic therapy to control antibiotic-resistant bacteria. Open Research DATA AVAILABILITY STATEMENT The data that support the findings of this study are available upon the request. Citing Literature Volume67, Issue6November 2020Pages 2809-2817 RelatedInformation