生物
神经嵴
间充质
斑马鱼
Hox基因
胚胎干细胞
脊椎动物
干细胞
细胞生物学
电池类型
命运图
祖细胞
细胞分化
神经干细胞
Wnt信号通路
细胞
基因
遗传学
胚胎
基因表达
信号转导
作者
Aubrey G.A. Howard,Phillip A Baker,Rodrigo Ibarra‐García‐Padilla,Joshua Moore,Lucia J Rivas,James J. Tallman,Eileen W. Singleton,Jessa L Westheimer,Julia A Corteguera,Rosa A. Uribe
出处
期刊:eLife
[eLife Sciences Publications, Ltd.]
日期:2021-02-16
卷期号:10
被引量:37
摘要
Neural crest cells (NCCs) are vertebrate stem cells that give rise to various cell types throughout the developing body in early life. Here, we utilized single-cell transcriptomic analyses to delineate NCC-derivatives along the posterior developing vertebrate, zebrafish, during the late embryonic to early larval stage, a period when NCCs are actively differentiating into distinct cellular lineages. We identified several major NCC/NCC-derived cell-types including mesenchyme, neural crest, neural, neuronal, glial, and pigment, from which we resolved over three dozen cellular subtypes. We dissected gene expression signatures of pigment progenitors delineating into chromatophore lineages, mesenchyme cells, and enteric NCCs transforming into enteric neurons. Global analysis of NCC derivatives revealed they were demarcated by combinatorial hox gene codes, with distinct profiles within neuronal cells. From these analyses, we present a comprehensive cell-type atlas that can be utilized as a valuable resource for further mechanistic and evolutionary investigations of NCC differentiation.
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