嗜酸性粒细胞
骨髓
免疫学
细胞毒性T细胞
生物
背景(考古学)
趋化性
白细胞介素5
癌症研究
白细胞介素
细胞因子
体外
受体
生物化学
古生物学
哮喘
作者
Sharon Grisaru,Michal Itan,Ariel Munitz
出处
期刊:Methods in molecular biology
日期:2021-01-01
卷期号:: 89-97
被引量:1
标识
DOI:10.1007/978-1-0716-1095-4_8
摘要
Eosinophils are bone marrow-derived cells that differentiate in the bone marrow and migrate into the peripheral blood primarily under the regulation of interleukin (IL)-5. Despite the fact that eosinophils have been mostly studied in the context of allergic inflammatory diseases, eosinophils accumulate in multiple tumors. In fact, recent data highlight key anti-tumorigenic activities for eosinophils. Thus, developing simple assays that will dissect the interactions between eosinophils and tumor cells is important since these assays will provide tools to study eosinophils in the tumor microenvironment. In this chapter, we provide detailed methods for isolating eosinophils from Il5 transgenic mice. Furthermore, we provide methodology to assess eosinophil chemotaxis in response to tumor-secreted factors. Finally, we describe a co-culture system of eosinophils and tumor cells aimed to determine the cytotoxic capabilities of eosinophils.
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