细胞生物学
MFN2型
生发泡
卵母细胞
线粒体
生物
主轴杆体
主轴装置
减数分裂
染色体分离
线粒体融合
中期
线粒体DNA
遗传学
染色体
胚胎
细胞分裂
细胞
基因
作者
Xing-Hua Wang,Shen Yin,Xiang‐Hong Ou,Shi‐Ming Luo
标识
DOI:10.1016/j.bbrc.2020.05.037
摘要
During oocyte meiosis, mitochondria usually surround spindle to meet the energy demand of spindle migration and chromosome segregation. Therefore, the mitochondrion surrounding spindle is widely accepted as an important indicator to demonstrate the mitochondrial function in oocyte studies. However, the role of mitochondria surrounding spindle in oocyte quality is not exactly addressed. Mitofusin-2 (MFN2) is a mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion. Here, we increased the mitochondria surrounding spindle by overexpression of MFN2 in mouse oocytes. Results indicate that the increase of mitochondria surrounding spindle has little effect on germinal vesicle breakdown (GVBD), spindle migration, mitochondrial membrane potential (MMP), reactive oxygen species (ROS) production and Endoplasmic reticulum (ER) distribution, while blocks chromosome segregation, destroys the spindle, and finally causes most of the oocytes to arrest at metaphase I stage. Collectively, our results demonstrate the mitochondria surrounding spindle is precisely regulated during oocyte maturation, while too much of it may cause abnormal oocyte meiosis. Therefore, although mitochondrion surrounding spindle is a typical biological event during oocyte maturation, utilizing it to demonstrate the mitochondrial function and oocyte quality should be much careful.
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