重组DNA
伴侣(临床)
生物化学
蛋白质折叠
靶蛋白
化学
细胞生物学
生物
计算生物学
基因
医学
病理
作者
Shweta Sharma,Roop Singh Bora,Kulvinder Singh Saini,Ranjana Arya
出处
期刊:Methods in molecular biology
日期:2022-01-01
卷期号:: 339-358
标识
DOI:10.1007/978-1-0716-1859-2_20
摘要
In the last two decades, numerous innovative advances, strategies and protocols have been developed and optimized to improve the quality and quantity of soluble recombinant protein production in E. coli. One of the major challenges being the coelution of chaperone proteins along with desired recombinant protein of interest. The removal of chaperones is important for protein yield, structural determination, optimal activity, and desired function of the recombinant protein. In this chapter, we outline various strategies for removal of chaperone contaminants from oligomeric proteins, with the ultimate objective of ameliorating the quality and proper folding of recombinant proteins. We have discussed in detail the purification and expression of full-length protein, GNE (UDP-N-acetylglucosamine 2-epimerase/ N-acetylmannosamine kinase), as a case study for chaperone removal.
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