滚动圆复制
环介导等温扩增
核酸
生物传感器
聚合酶链反应
DNA
PCR的应用
计算生物学
多重位移放大
模板
聚合酶
纳米技术
生物
材料科学
生物化学
DNA提取
基因
数字聚合酶链反应
作者
Yongping Gao,Ke‐Jing Huang,Futing Wang,Yangyang Hou,Jing Xu,Guoqiang Li
出处
期刊:Analyst
[The Royal Society of Chemistry]
日期:2022-01-01
卷期号:147 (15): 3396-3414
被引量:26
摘要
Rolling circle amplification (RCA) is a simple and isothermal DNA amplification technique that is used to generate thousands of repeating DNA sequences using circular templates under the catalysis of DNA polymerase. Compared to alternating temperature nucleic acid amplification such as polymerase chain reaction (PCR) amplification, RCA is more suitable for on-spot detection without the need for an expensive thermal cycler. In this study, the principle and classification of RCA are introduced, and the applications of RCA in the detection of pathogenic bacteria, nucleic acid tumor markers, viruses, and proteins are reviewed. Finally, the perspectives of RCA in biological detection are anticipated. The RCA method has a great potential for biological detection. This review aims to provide references for the further development and application of the RCA technique in biosensors.
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