光形态发生
生物
植物
遗传学
拟南芥
基因
突变体
作者
Ruohe Yin,Ling Li,Kaijing Zuo
出处
期刊:Methods in molecular biology
日期:2021-01-01
被引量:3
标识
DOI:10.1007/978-1-0716-1370-2
摘要
This book aims at providing readers with protocols for research in plant photomorphogenesis.The chapters in this book include a broad range of topics, including assays for shade avoidance responses, assays for light-dependent protein-protein interactions, photobody detection with immunofluorescence and the super-resolution imaging method, photoreceptor containing protein complex isolation from plants, detection of homodimer and monomer of photoreceptor UVR8 with immunoblotting analysis, assays for seedling greening, procedures for studying skotomorphogenesis, phenotypic study of photomorphogenesis at seedling stage, expression of cryptochrome in insect cells, and more.Each chapter includes a list of the necessary materials and reagents, detailed reproducible experimental procedures, and notes on avoiding known pitfalls.This book, Plant Photomorphogenesis: Methods and Protocols, serves as a guideline to researchers or students who are new to the field, and a stepping-stone for experienced researchers to further their skills in this fast-developing field.
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