Facile immobilization of glucose oxidase with Cu3(PO4)2·3H2O for glucose biosensing via smartphone

A smartphone-based colorimetric platform for facile glucose detection was constructed below. First, glucose oxidase-Cu3(PO4)2·3H2O hybrid microflowers (GOx-HMFs) were facilely synthesized via biomineralization, which can react with glucose and 3,3',5,5'-tetramethylbenzidine to generate colored product. Next, the color information of product was real-time collected and processed via smartphone to realize accurate glucose detection. The as-constructed colorimetric platform based on GOx-HMFs and smartphone had wide linear range, high sensitivity and good selectivity for glucose detection, moreover, the detection process was convenient and efficient, which provided a new idea for glucose detection.