化学
色谱法
咖啡酸
串联质谱法
串联
质谱法
液相色谱-质谱法
有机化学
抗氧化剂
材料科学
复合材料
作者
Shang‐Gao Liao,Lijuan Zhang,Cui‐Bing Li,Yan‐Yu Lan,Aimin Wang,Yong Huang,Zhen Lin,Xiao‐Zhong Fu,Wen Zhou,Xiaolan Qi,Zhi‐Zhong Guan,Yonglin Wang
摘要
Abstract Caffeic acid and its esters (CAEs) are widely distributed in the plant kingdom and have been reported to elicit a wide range of exceptional biological activities. Present methods for screening and characterization of CAEs normally need the use of liquid chromatography diode‐array detection/multistage mass spectrometry (LC‐DAD/MS n ). In this report, a rapid and efficient method coupling ultra‐performance liquid chromatography (UPLC) with fragment‐targeted multi‐reaction monitoring (MRM) has been developed for screening CAEs in a crude extract of Erigeron breviscapus , while a UPLC/quasi‐MS n method has been applied in the structural identification of these compounds. Furthermore, a simple quasi‐UPLC/MS/MS method based on in‐source collision‐induced dissociation (CID) has been proposed for rapid identification of the CAEs. As a result, a total of more than 34 CAEs were detected and their structures characterized. Nine of them were reported from E. breviscapus for the first time. Applications of these strategies in the chemical investigation of an injection of E. breviscapus resulted in the identifications of 16 CAEs. These strategies, if appropriate modifications are made, will be very useful in screening and characterization not only of CAEs, but of other structural types of compounds in various complex matrices. Copyright © 2010 John Wiley & Sons, Ltd.
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