丹参
克隆(编程)
RNA干扰
生物
基因
遗传学
适配器(计算)
转录因子
基因沉默
计算生物学
分子生物学
计算机科学
核糖核酸
医学
替代医学
病理
中医药
程序设计语言
操作系统
出处
期刊:China journal of Chinese materia medica
[China Journal of Chinese Materia Medica]
日期:2014-05-01
标识
DOI:10.4268/cjcmm20140905
摘要
NAC transcription factors involved in plant growth and development, as well as responses to biotic and abiotic stress. RNAi Vectors for SmNAC transcription factors of Salvia miltiorrhiza was constructed by using Gateway cloning technology, in order to further study the function of SmNAC1 transcription factor. According to Gateway cloning technology, the specific fragments of SmNAC1 containing attB adapter was amplified by PCR using ultra-fideling phusion polymerase of NEB. By the BP recombination reaction, the PCR product containing attB was transferred to an donor vector (pENTR/SD/D-TOPO). Finally, SmNACi specific gene was cloned into pK7GWIWG2D plant expression vectors by LR recombination reaction. Experimental results showed that Gateway cloning technology provide a rapid and highly efficient way to clone the interested gene.
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