Dynamics between horizontal gene transfer and acquired antibiotic resistance inS. Heidelberg followingin vitroincubation in broiler ceca

生物 微生物学 盲肠 质粒 肠沙门氏菌 水平基因转移 抵抗性 抗生素耐药性 微生物群 基因 流动遗传元素 毒力 细菌 遗传学 大肠杆菌 抗生素 基因组 生态学
作者
Adelumola Oladeinde,Kimberly Cook,Steven M. Lakin,Zaid Abdo,Torey Looft,Kyler Herrington,Gregory Zock,Jodie Plumblee Lawrence,Jesse C. Thomas,Marie-Christine Beaudry,Travis C. Glenn
标识
DOI:10.1101/684787
摘要

Abstract The chicken gastrointestinal tract harbors taxa of microorganisms that play a role in the health and disease status of the host. The cecum is the part of the gut that carries the highest microbial densities, has the longest residence time of digesta and is a vital site for urea recycling and water regulation. Therefore, the cecum provides a rich environment for bacteria to horizontally transfer genes between one another via mobile genetic elements such as plasmids and bacteriophages. In this study, we used broiler chicken cecum as a model to investigate antibiotic resistance genes that can be transferred in vitro from ceca flora to Salmonella enterica serovar Heidelberg ( S . Heidelberg). We used whole genome sequencing and resistome enrichment to decipher the interactions between S . Heidelberg, gut microbiome and acquired antibiotic resistance. After 48 h incubation of ceca under microaerophilic conditions, one S . Heidelberg isolate was recovered with an acquired Inck2 plasmid (88 kb) encoding extended β-lactamase producing gene ( bla CMY-2 ). In vitro , this plasmid was transferrable between E. coli and S . Heidelberg strains, but transfer was unsuccessful between S . Heidelberg strains. An in-depth genetic characterization of transferred plasmids suggests that they share significant homology with P1-like phages. This study contributes to our understanding of the dynamics between an important food-borne pathogen and the chicken gut microbiome. Importance S. Heidelberg is a clinically important serovar, linked to food-borne illness and among the top 5 serovars isolated from poultry in USA and Canada. Acquisition of new genetic material from microbial flora in the gastrointestinal tract of food animals, including broilers, may contribute to increased fitness of pathogens like S. Heidelberg and may increase their level of antibiotic tolerance. Therefore, it is critical to gain a better understanding on the dynamic interactions that occur between important pathogens and the commensals present in the animal gut and other agroecosystems. In this study, we show that the native flora in the broiler ceca were capable of transferring mobile genetic elements carrying AmpC β-lactamase ( bla CMY-2 ) gene to an important food-borne pathogen S . Heidelberg. The potential role for P1-like bacteriophage transduction was also discussed.
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