适体
电化学发光
检出限
生物传感器
化学
生物分析
纳米技术
滚动圆复制
组合化学
DNA
色谱法
分子生物学
生物化学
材料科学
DNA复制
生物
作者
Yamin Nie,Xiaoding Yuan,Pu Zhang,Yaqin Chai,Ruo Yuan
标识
DOI:10.1021/acs.analchem.8b05001
摘要
Electrochemiluminescence (ECL), as a sensitive and controllable assay, offers a considerable opportunity for multiple types of biomarkers detection. However, constructing such a biosensor remains a significant challenge. Herein, an ultrasensitive and versatile ECL biosensor was constructed to detect multiple types of biomarkers from breast cancer by taking the strategies of nonenzymatic catalytic hairpin assembly (CHA) and hybridization chain reaction (HCR) amplification, as well as aptamer-triggered emitter release. Concretely, with the appearance of target 1 microRNA-21 (miRNA-21), abundant double-stranded DNA (dsDNA) polymers were generated on this biosensing surface via amplification circuits of CHA and HCR, which could be intercalated into substantial ([Ru(bpy)2dppz]Cl2) as ECL indicators to obtain an obvious enhancement of ECL signal for target 1 detection with a detection limit (0.1 fM). Furthermore, in the presence of target 2 human mucin 1 (MUC1) protein, the ECL signal had a distinct decrease, because aptamer recognition induced the release of [Ru(bpy)2dppz]Cl2 from the sensing surface, thus, achieving a sensitive detection for MUC1 with a detection limit (2.4 fg·mL-1). Simultaneously, this sensing platform was applied to monitor the biomarkers from MDA-MB-231 breast cancer cells, suggesting that this method was applicable to detect real samples. Therefore, this platform is an applicable and versatile implement for the determination of multiple types of biomarkers to improve diagnostic accuracy and efficiency.
科研通智能强力驱动
Strongly Powered by AbleSci AI