Nitrogen- and sulfur-doped carbon dots as peroxidase mimetics: colorimetric determination of hydrogen peroxide and glutathione, and fluorimetric determination of lead(II)

Fluorescent carbon dots co-doped with nitrogen and sulfur (N/S CDs) were prepared and found to display viable peroxidase mimicking activity. They have a blue fluorescence (with excitation/emission maxima at 340/456 nm) with a quantum yield of 35%. The N/S CDs catalyze the oxidation of 3,3,5,5-tetramethylbenzidine (TMB) in the presence of H2O2, and this leads to the appearance of a blue solution with a absorption maximum at 654 nm. A colorimetric method was developed for the determination of H2O2 that has a 1.75 μM detection limit and a linear response in the 10−5 to 10−4 M concentration range. The method can be extended to the enzymatic determination of glutathione with a 0.26 μM detection limit and a working range from 0.20 to 100 μM. In addition, the CDs respond to lead(II) which is a quencher of the blue fluorescence at 456 nm, with a detection limit of 11 μM and a working range up to 100 μM. Simultaneously, the color changes can be visually detected with absorbance signal changes from 10 to 100 μM with limit of 3.9 μM. A multiple detection system was worked out that allows monitoring of H2O2 and glutathione successively, and of lead(II). (A) Schematic representation of the nitrogen & sulphur doped carbon dots with blue fluorescence, (B) the peroxidase-like activity in colorimetric detecting of H2O2 and GSH and (C) the illustration for the application of Pb2+ detection with fluorescence and colorimetric method.