荧光相关光谱
分子间力
化学物理
扩散
分子
航程(航空)
化学
光谱学
布朗动力学
荧光
布朗运动
分子动力学
水动力半径
静电学
力谱学
离子强度
半径
计算化学
材料科学
物理化学
热力学
物理
水溶液
光学
量子力学
有机化学
复合材料
计算机科学
胶束
计算机安全
作者
Ilaria Nardecchia,Mathias Lechelon,Matteo Gori,Irene Donato,Jordane Preto,Elena Floriani,Sébastien Jaeger,Sébastien Mailfert,Didier Marguet,Pierre Ferrier,Marco Pettini
出处
期刊:Physical review
日期:2017-08-09
卷期号:96 (2)
被引量:15
标识
DOI:10.1103/physreve.96.022403
摘要
The present paper deals with an experimental feasibility study concerning the detection of long- range intermolecular interactions through molecular diffusion behavior in solution. This follows previous analyses, theoretical and numerical, where it was found that inter-biomolecular long-range force fields of electrodynamic origin could be detected through deviations from Brownian diffusion. The suggested experimental technique was Fluorescence Correlation Spectroscopy (FCS). By con- sidering two oppositely charged molecular species in watery solution, that is, Lysozyme protein and a fluorescent dye molecule (Alexa488), the diffusion coefficient of the dye has been measured by means of the FCS technique at different values of the concentration of Lysozyme molecules, that is, at different average distances between the oppositely charged molecules. For the model consid- ered long-range interactions are built-in as electrostatic forces, the action radius of which can be varied by changing the ionic strength of the solution. The experimental outcomes clearly prove the detectability of long-range intermolecular interactions by means of the FCS technique. Molecular Dynamics simulations provide a clear and unambiguous interpretation of the experimental results.
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