酵母
溶解循环
质粒
聚合酶链反应
生物
基因组DNA
DNA
多重位移放大
基因组
微生物学
分子生物学
化学
遗传学
基因
DNA提取
病毒
作者
Juan I. Fuxman Bass,John S. Reece‐Hoyes,Albertha J.M. Walhout
出处
期刊:CSH Protocols
[Cold Spring Harbor Laboratory]
日期:2016-12-01
卷期号:2016 (12): pdb.prot088971-pdb.prot088971
被引量:15
标识
DOI:10.1101/pdb.prot088971
摘要
Here, we present a protocol for amplifying DNA fragments from the genome of, or plasmids transformed into, yeast strains that require the use of the lytic enzyme zymolyase to break open the yeast cells by digesting the cell wall. Yeast strains requiring such treatment include YM4271 and Y1HaS2, whereas other yeast strains (e.g., MaV103) might not require treatment with Zymolyase.
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