Characterization of the 12q amplicons in lipomatous soft tissue tumors by multiplex ligation-dependent probe amplification-based copy number analysis.

放大器 生物 脂肪肉瘤 癌症研究 基因 多重连接依赖探针扩增 分子生物学 病理 遗传学 肉瘤 医学 聚合酶链反应 外显子
作者
David Creytens,Joost van Gorp,Ernst‐Jan M. Speel,Liesbeth Ferdinande
出处
期刊:PubMed 卷期号:35 (4): 1835-42 被引量:16
链接
标识
摘要

Well-differentiated liposarcoma (WDLPS) and de-differentiated liposarcoma (DDLPS) are characterized by amplified sequences derived from the long arm of chromosome 12. The goal of the present study was to identify, besides the well-known candidate genes, novel relevant genes in these large, complex 12q amplicons.Using multiplex ligation-dependent probe amplification, genetic alterations in 19 different genes of 12q12-24 were evaluated in 77 lipomatous soft tissue tumors (including lipomas, WDLPS, DDLPS and pleomorphic liposarcomas).We recorded several amplified genes of 12q13-15, including miR-26a-2, a gene not well studied in liposarcoma, and the well-known and previously described genes murine double minute 2 (MDM2), YEATS domain-containing protein 4 (YEATS4), high-mobility AT-hook 2 (HMGA2), cyclin-dependent kinase 4 (CDK4) and tetraspanin 31 (TSPAN31). Interestingly, the amplification profiles of these six genes were found to be significantly different between WDLPS and DDLPS, more frequently having a high-level status in DDLPS than in WDLPS. In addition, DDLPS were found to have significantly higher mean amplification ratios compared to WDLPS. Moreover, we identified additional genes exclusively amplified in DDLPS in 12q13, 12q21 and 12q24, including glioma-associated oncogene homolog 1 (GLI1), mitogen activated protein kinase kinase kinase 12 (MAP3K12), cyclin-dependent kinase 2 (CDK2), ALX homeobox 1 (ALX1) and T-box 5 (TBX5).Differences in amplification profiles among WDLPS and DDLPS may be related to progression/de-differentiation in liposarcomas and show how in the future amplification profiles could provide an adjunctive tool in characterizing progression to DDLPS. In addition, we identified additional genes exclusively amplified in DDLPS, which may play a role in liposarcomagenesis, particularly in the de-differentiation process.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
打打应助hino采纳,获得10
刚刚
小星完成签到 ,获得积分10
刚刚
zuozuo完成签到,获得积分10
刚刚
小鬼丶发布了新的文献求助10
1秒前
橘子完成签到,获得积分10
1秒前
2秒前
苑阿宇完成签到 ,获得积分10
2秒前
惰性发布了新的文献求助10
3秒前
3秒前
4秒前
东方耀发布了新的文献求助10
4秒前
小鬼丶完成签到,获得积分10
5秒前
ljh完成签到 ,获得积分10
5秒前
schyoung完成签到,获得积分10
5秒前
1989完成签到,获得积分10
5秒前
7秒前
CipherSage应助小鬼丶采纳,获得10
7秒前
但大图完成签到 ,获得积分10
8秒前
天天发布了新的文献求助10
8秒前
大媛大靳吃地瓜完成签到,获得积分10
8秒前
小鱼完成签到 ,获得积分10
8秒前
when发布了新的文献求助10
9秒前
你的小可爱突然出现完成签到,获得积分10
15秒前
大个应助爱听歌笑寒采纳,获得10
15秒前
16秒前
无花果应助parpate采纳,获得10
17秒前
Hellolyj应助yue采纳,获得10
18秒前
摆烂完成签到 ,获得积分10
18秒前
王崇然完成签到,获得积分10
20秒前
geen完成签到,获得积分10
21秒前
BOSS徐应助东方耀采纳,获得10
21秒前
dongyu发布了新的文献求助10
22秒前
风起青禾完成签到,获得积分10
22秒前
执着以山完成签到,获得积分10
24秒前
24秒前
jz完成签到,获得积分10
25秒前
Hello完成签到,获得积分20
25秒前
26秒前
单纯夏云发布了新的文献求助10
26秒前
隐形蛋挞完成签到,获得积分20
27秒前
高分求助中
Evolution 10000
Sustainability in Tides Chemistry 2800
юрские динозавры восточного забайкалья 800
English Wealden Fossils 700
An Introduction to Geographical and Urban Economics: A Spiky World Book by Charles van Marrewijk, Harry Garretsen, and Steven Brakman 600
Diagnostic immunohistochemistry : theranostic and genomic applications 6th Edition 500
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 400
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3154330
求助须知:如何正确求助?哪些是违规求助? 2805172
关于积分的说明 7863751
捐赠科研通 2463360
什么是DOI,文献DOI怎么找? 1311251
科研通“疑难数据库(出版商)”最低求助积分说明 629543
版权声明 601821