Colorimetric Detection of Cholesterol Based on the Peroxidase‐Like Activity of Metal‐Organic Framework MIL‐101(Cr)

Abstract Efficient quantitative determination of cholesterol has important application value in drug testing and clinical diagnosis of cardiovascular diseases. In this work, a metal‐organic framework MIL‐101(Cr) (MIL=Materials of Institute Lavoisier) was synthesized and developed as a peroxidase mimic for colorimetric detection of cholesterol. Kinetic analysis indicated that the MIL‐101(Cr) nanoparticles showed a higher V max (2.27*10 −7 MS −1 ) and a lower K m (0.07 mM) than the natural horseradish peroxidase (HRP). An effective colorimetric sensor has been proven to detect cholesterol. Based on the outstanding catalytic activity, the colorimetric sensor displayed a linear response for cholesterol in the concentration range from 1.1 μM to 22.9 μM and the detection limit as low as 0.86 μM. Furthermore, the proposed method based on MIL‐101(Cr) nanoparticles showed a favorable reproducibility and recovery. The sensor had been successfully applied to determine serum cholesterol level in mice and human, respectively.