诱导多能干细胞
细胞生物学
干细胞
软骨
骨愈合
类有机物
生物
解剖
胚胎干细胞
生物化学
基因
作者
Wai Long Tam,Luís Freitas Mendes,Xike Chen,R Lesage,Inge Van Hoven,Elke Leysen,Greet Kerckhofs,Kathleen Bosmans,Yoke Chin Chai,Akihiro Yamashita,Noriyuki Tsumaki,Liesbet Geris,Scott J. Roberts,Frank P. Luyten
标识
DOI:10.1186/s13287-021-02580-7
摘要
Bones have a remarkable capacity to heal upon fracture. Yet, in large defects or compromised conditions healing processes become impaired, resulting in delayed or non-union. Current therapeutic approaches often utilize autologous or allogeneic bone grafts for bone augmentation. However, limited availability of these tissues and lack of predictive biological response result in limitations for clinical demands. Tissue engineering using viable cell-based implants is a strategic approach to address these unmet medical needs.Herein, the in vitro and in vivo cartilage and bone tissue formation potencies of human pluripotent stem cells were investigated. The induced pluripotent stem cells were specified towards the mesodermal lineage and differentiated towards chondrocytes, which subsequently self-assembled into cartilaginous organoids. The tissue formation capacity of these organoids was then challenged in an ectopic and orthotopic bone formation model.The derived chondrocytes expressed similar levels of collagen type II as primary human articular chondrocytes and produced stable cartilage when implanted ectopically in vivo. Upon targeted promotion towards hypertrophy and priming with a proinflammatory mediator, the organoids mediated successful bridging of critical size long bone defects in immunocompromised mice.These results highlight the promise of induced pluripotent stem cell technology for the creation of functional cartilage tissue intermediates that can be explored for novel bone healing strategies.
科研通智能强力驱动
Strongly Powered by AbleSci AI