亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Profiling and Characterization of microRNAs Responding to Sodium Butyrate Treatment in Gastric Cancer Cells

丁酸钠 小RNA 丁酸盐 癌症研究 细胞凋亡 小桶 癌症 生物 化学 分子生物学 癌细胞 计算生物学 细胞生物学 生物化学 细胞 基因 基因表达 转录组 遗传学 发酵
作者
Dewei Zhang,Gongping Sun,He Duan,Jin Meng
出处
期刊:Combinatorial Chemistry & High Throughput Screening [Bentham Science Publishers]
卷期号:25 (11): 1875-1888 被引量:4
标识
DOI:10.2174/1386207325666211027154207
摘要

Short-chain fatty acids exert anti-cancer effects on tumor cells.We aimed to reveal the signaling network altered by butyrate in Gastric Cancer (GC) using small RNA sequencing (sRNA-seq).The effects of butyrate on the biological behavior of NCI-N87 and KATO III cells in vitro were assessed by functional assays and half-maximal inhibitory concentrations (IC50) of butyrate in KATO III cells were calculated. sRNA-seq was performed on KATO III cells. Differentially expressed miRNAs (DE-miRNAs) were identified between butyrate treatment and control groups using DESeq2, and miRNA targets were predicted. A protein-protein interaction (PPI) network of DE-miRNA targets was created using Metascape. Key MCODE complexes were identified using the MCODE algorithm and cluster Profiler. The relationship between DE-miRNA and GC overall survival (OS) was evaluated using Kaplan-Meier curves.Butyrate dose-dependently inhibited NCI-N87 and KATO III cell viability. KATO III cells were more sensitive to butyrate than NCI-N87 cells. Butyrate promoted apoptosis and inhibited KATO III cell migration. Total 324 DE-miRNAs were identified in KATO III cells, and 459 mRNAs were predicted as targets of 83 DE-miRNAs. Two key protein complexes were identified in a PPI network of the 459 targets. A key signaling network responding to butyrate was generated using targets in these key complexes and their miRNA regulators. The DE-miRNAs in the key signaling network were related to the OS of GC.Butyrate altered the biological behavior of GC cells, which may be achieved by regulating miRNAs and related oncogenic pathways.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
笨笨善若发布了新的文献求助10
5秒前
Tristan完成签到 ,获得积分10
7秒前
唠叨的傲薇完成签到 ,获得积分10
9秒前
笨笨善若完成签到,获得积分10
11秒前
夏瑞发布了新的文献求助30
14秒前
深情安青应助栗栗栗子采纳,获得10
18秒前
19秒前
19秒前
拾光完成签到,获得积分10
23秒前
哈哈发布了新的文献求助10
26秒前
FrozNineTivus完成签到,获得积分10
26秒前
兜兜完成签到 ,获得积分10
28秒前
哈哈完成签到,获得积分10
52秒前
夏瑞完成签到,获得积分10
55秒前
wangyang完成签到 ,获得积分10
55秒前
闪闪蜜粉完成签到 ,获得积分10
1分钟前
1分钟前
1分钟前
lailai完成签到 ,获得积分10
1分钟前
cgg完成签到 ,获得积分10
1分钟前
迷你的靖雁完成签到,获得积分10
1分钟前
1分钟前
1分钟前
小L完成签到 ,获得积分10
1分钟前
核桃发布了新的文献求助10
1分钟前
斜阳完成签到 ,获得积分10
1分钟前
xiloyou完成签到 ,获得积分10
1分钟前
大个应助科研通管家采纳,获得10
1分钟前
luna完成签到,获得积分10
2分钟前
2分钟前
2分钟前
2分钟前
2分钟前
lsm发布了新的文献求助10
2分钟前
2分钟前
2分钟前
和谐诗双完成签到 ,获得积分10
2分钟前
obsidian发布了新的文献求助10
2分钟前
2分钟前
高分求助中
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3990012
求助须知:如何正确求助?哪些是违规求助? 3532077
关于积分的说明 11256227
捐赠科研通 3270933
什么是DOI,文献DOI怎么找? 1805139
邀请新用户注册赠送积分活动 882270
科研通“疑难数据库(出版商)”最低求助积分说明 809228