巨核细胞
透射电子显微镜
骨髓
细胞生物学
细胞质
核心
超微结构
血小板
细胞外
原位
膜
生物
化学
病理
生物物理学
材料科学
免疫学
解剖
造血
生物化学
纳米技术
医学
有机化学
干细胞
作者
Cyril Scandola,François Lanza,Christian Gachet,Anita Eckly
摘要
Differentiation and maturation of megakaryocytes occur in close association with the cellular and extracellular components of the bone marrow. These processes are characterized by the gradual appearance of essential structures in the megakaryocyte cytoplasm such as a polyploid and polylobulated nucleus, an internal membrane network called demarcation membrane system (DMS) and the dense and alpha granules that will be found in circulating platelets. In this article, we describe a standardized protocol for the in situ ultrastructural study of murine megakaryocytes using transmission electron microscopy (TEM), allowing for the identification of key characteristics defining their maturation stage and cellular density in the bone marrow. Bone marrows are flushed, fixed, dehydrated in ethanol, embedded in plastic resin, and mounted for generating cross-sections. Semi-thin and thin sections are prepared for histological and TEM observations, respectively. This method can be used for any bone marrow cell, in any EM facility and has the advantage of using small sample sizes allowing for the combination of several imaging approaches on the same mouse.
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