Effects of the ambient fine particulate matter (PM2.5) exposure on urinary metabolic profiles in rats using UPLC-Q-TOF-MS

化学 葡萄糖醛酸盐 代谢组学 代谢途径 新陈代谢 代谢物 代谢组 色谱法 马尿酸 生物化学 尿
作者
Lin Zhang,Tengfei Xu,Zifeng Pi,Meizhu Zheng,Fengrui Song,Zhiqiang Liu
出处
期刊:Chinese Chemical Letters [Elsevier BV]
卷期号:30 (1): 90-94 被引量:13
标识
DOI:10.1016/j.cclet.2017.11.019
摘要

In order to study the damage of ambient fine particulate matter (PM2.5) to organism in the environment, the method of urinary metabolomics was used to detect the potential endogenous metabolites and their related metabolic pathways to clarify the toxicity mechanisms. Particles of PM2.5 collected from the fibrous membrane were extracted ultrasonically and dissolved in physiological saline to prepare PM2.5 suspension. The solution of saline and fine particular matter was injected into the trachea of rats respectively. The rats were injected two times a week for four weeks. Samples of 24-h urine were collected at the 14th day after the end of exposure, and the ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) was used for the metabolomics detection. Principal component analysis (PCA) was used to investigate the global metabolomic alterations and a clear separation in the scatter diagram were observed. 17 potential endogenous metablites were identified from urinary samples in rats by UPLC-Q-TOF-MS. The primary metabolism pathways involved pentose and glucuronate interconversions, starch and sucrose metabolism, tryptophan metabolism, tyrosine metabolism, phenylalanine metabolism, purine metabolism, acetaminophen metabolism pathway, retinol metabolism and valproic acid metabolism pathway. The results are helpful to understand the toxicological mechanisms of PM2.5 and screened out potential biomarkers of rats which exposure to PM2.5.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
研友_VZG7GZ应助研友_Z33zkZ采纳,获得10
刚刚
小二郎应助体贴向日葵采纳,获得10
1秒前
2秒前
wyz完成签到,获得积分10
3秒前
3秒前
Woshikeyandawang完成签到,获得积分10
4秒前
完美世界应助fvsd采纳,获得10
6秒前
6秒前
YI完成签到,获得积分10
6秒前
阿航完成签到,获得积分10
7秒前
wyz发布了新的文献求助10
7秒前
进步完成签到,获得积分10
8秒前
Fluoxetine完成签到,获得积分10
8秒前
queer发布了新的文献求助20
8秒前
蘑菇丰收发布了新的文献求助150
8秒前
fan发布了新的文献求助10
8秒前
Ayers完成签到,获得积分10
9秒前
调皮初蝶发布了新的文献求助10
9秒前
ll应助科研通管家采纳,获得10
11秒前
天天快乐应助科研通管家采纳,获得10
11秒前
11秒前
顾矜应助科研通管家采纳,获得30
11秒前
yar应助科研通管家采纳,获得10
11秒前
科研通AI5应助科研通管家采纳,获得10
11秒前
yar应助科研通管家采纳,获得10
11秒前
小二郎应助科研通管家采纳,获得10
11秒前
11秒前
隐形曼青应助Ayers采纳,获得10
12秒前
Connor关注了科研通微信公众号
13秒前
ED应助进步采纳,获得10
14秒前
莫言发布了新的文献求助30
14秒前
白鹤想睡觉完成签到,获得积分10
15秒前
16秒前
17秒前
17秒前
搁浅完成签到 ,获得积分10
17秒前
CFJ完成签到,获得积分10
18秒前
徐志成发布了新的文献求助10
19秒前
19秒前
NexusExplorer应助不吃西瓜采纳,获得10
20秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 700
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3975378
求助须知:如何正确求助?哪些是违规求助? 3519775
关于积分的说明 11199621
捐赠科研通 3256067
什么是DOI,文献DOI怎么找? 1798124
邀请新用户注册赠送积分活动 877386
科研通“疑难数据库(出版商)”最低求助积分说明 806305