丹参
二萜
萜类
化学
转基因
生物化学
毛状根培养
拟南芥
ATP合酶
质体
转基因作物
植物
基因
生物
农杆菌
叶绿体
突变体
替代医学
中医药
病理
医学
作者
Min Shi,Xiuqin Luo,Guanhua Ju,Leilei Li,Sheng‐Xiong Huang,Tong Zhang,Huizhong Wang,Guoyin Kai
标识
DOI:10.1021/acs.jafc.5b04697
摘要
Tanshinones are health-promoting diterpenoids found in Salvia miltiorrhiza and have wide applications. Here, SmGGPPS (geranylgeranyl diphosphate synthase) and SmDXSII (1-deoxy-d-xylulose-5-phosphate synthase) were introduced into hairy roots of S. miltiorrhiza. Overexpression of SmGGPPS and SmDXSII in hairy roots produces higher levels of tanshinone than control and single-gene transformed lines; tanshinone production in the double-gene transformed line GDII10 reached 12.93 mg/g dry weight, which is the highest tanshinone content that has been achieved through genetic engineering. Furthermore, transgenic hairy root lines showed higher antioxidant and antitumor activities than control lines. In addition, contents of chlorophylls, carotenoids, indoleacetic acid, and gibberellins were significantly elevated in transgenic Arabidopsis thaliana plants. These results demonstrate a promising method to improve the production of diterpenoids including tanshinone as well as other natural plastid-derived isoprenoids in plants by genetic manipulation of the 2-C-methyl-d-erythritol-4-phosphate (MEP) pathway.
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